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Make the Frame Transparent
You can make the frame background transparent. This is useful if you want to make an animated GIF file that has a transparent background. Select a frame.
You will see the Frame Options panel.
Click the Fill
check box to turn off the frame background fill.
You will see a checkerboard background to indicate that the background is transparent.
Click the Apply to All Frames
button to make the background transparent for all of the frames.